Primer - 3
Always check your designed primers for secondary structure using OligoAnalyzer (IDT) or OligoCalc, and always run a gradient PCR to determine optimal annealing temperature the first time you test a new primer set.
Always check your designed primers for secondary structure using OligoAnalyzer (IDT) or OligoCalc, and always run a gradient PCR to determine optimal annealing temperature the first time you test a new primer set.