Uji Molisch Jurnal [exclusive] «COMPLETE • 2024»

Title: Application of the Molisch Test for the Rapid Detection of Carbohydrates in Unknown Analytes

Uji Molisch, α-naftol, karbohidrat, furfural, analisis kualitatif Note: If you need the full structure of a journal article (Introduction, Methods, Results, Discussion) instead of just an abstract, let me know and I will write that out for you. uji molisch jurnal

Molisch test, α-naphthol, carbohydrates, furfural, qualitative analysis Indonesian Version (Bahasa Indonesia) Judul: Uji Molisch untuk Identifikasi Karbohidrat secara Kualitatif Title: Application of the Molisch Test for the

The Molisch test, a general colorimetric reaction for carbohydrates, was performed to detect the presence of saccharides in various unknown samples. The test utilizes α-naphthol in an ethanolic solution (Molisch’s reagent) as the detection agent. Upon the addition of concentrated sulfuric acid, a purple-violet ring formed at the interface of the two liquids, indicating a positive result. The reaction mechanism relies on the dehydration of carbohydrates to furfural (from pentoses) or hydroxymethylfurfural (from hexoses), which subsequently condenses with α-naphthol to form a colored complex. All carbohydrate samples (monosaccharides, disaccharides, and polysaccharides) yielded positive results, while non-carbohydrate controls remained negative. The test demonstrated high sensitivity and simplicity, confirming its utility as a preliminary screening method for carbohydrate analysis in biochemical research. Upon the addition of concentrated sulfuric acid, a